Fly Brain Anatomy: FlyLight Gen1 and Split-GAL4 Imagery

Stream data with DDA:

from dagshub.streaming import DagsHubFilesystem

fs = DagsHubFilesystem(".", repo_url="https://dagshub.com/DagsHub-Datasets/janelia-flylight-dataset")

fs.listdir("s3://janelia-flylight-imagery")

Description:

This data set, made available by Janelia's FlyLight project, consists of fluorescence images of Drosophila melanogaster driver lines, aligned to standard templates, and stored in formats suitable for rapid searching in the cloud. Additional data will be added as it is published.

Contact:

This data set, made available by Janelia's FlyLight project, consists of fluorescence images of Drosophila melanogaster driver lines, aligned to standard templates, and stored in formats suitable for rapid searching in the cloud. Additional data will be added as it is published.

Update Frequency:

As required

Managed By:

https://www.janelia.org

Resources:

1. resource:

   • Description: Imagery and Metadata
   • ARN: arn:aws:s3:::janelia-flylight-imagery
   • Region: us-east-1
   • Type: S3 Bucket

2. resource:

   • Description: Alignment Templates
   • ARN: arn:aws:s3:::janelia-flylight-templates
   • Region: us-east-1
   • Type: S3 Bucket

3. resource:

   • Description: Color Depth MIPs
   • ARN: arn:aws:s3:::janelia-flylight-color-depth
   • Region: us-east-1
   • Type: S3 Bucket

Tags:

aws-pds, biology, life sciences, imaging, neuroscience, neurobiology, neuroimaging, fluorescence imaging, microscopy, image processing

Tutorials:

1. tutorial:

   • Title: File Operations on AWS S3
   • URL: https://github.com/JaneliaSciComp/open-data-flylight/blob/master/tutorials/File_operations_on_AWS_S3.ipynb
   • AuthorName: Rob Svirskas

2. tutorial:

   • Title: Using Imagery on AWS S3
   • URL: https://github.com/JaneliaSciComp/open-data-flylight/blob/master/tutorials/Using_imagery_on_AWS_S3.ipynb
   • AuthorName: Rob Svirskas

3. tutorial:

   • Title: Scaling Neuroscience Research on AWS
   • URL: https://aws.amazon.com/blogs/architecture/scaling-neuroscience-research-on-aws/
   • AuthorName: Konrad Rokicki
   • Services: Lambda, AppSync, DynamoDB, Step Functions

4. tutorial:

   • Title: Tutorial for neuronbridger (R API)
   • URL: http://natverse.org/neuronbridger/
   • AuthorName: Alexander Bates

Tools & Applications:

1. tools & applications:

   • Title: FlyLight Project Website
   • URL: https://www.janelia.org/project-team/flylight
   • AuthorName: Geoffrey Meissner

2. tools & applications:

   • Title: Fly Light Split-GAL4 Driver Collection
   • URL: http://splitgal4.janelia.org/cgi-bin/splitgal4.cgi
   • AuthorName: Rob Svirskas

3. tools & applications:

   • Title: Color Depth Search Fiji Plugin
   • URL: https://github.com/JaneliaSciComp/ColorMIP_Mask_Search
   • AuthorName: Hideo Otsuna

4. tools & applications:

   • Title: NeuronBridge
   • URL: https://neuronbridge.janelia.org
   • AuthorName: Jody Clements, Rob Svirskas, Hideo Otsuna, Cristian Goina, Konrad Rokicki

Publication:

1. publication:

   • Title: A GAL4-Driver Line Resource for Drosophila Neurobiology
   • URL: https://doi.org/10.1016/j.celrep.2012.09.011
   • AuthorName: Arnim Jenett, Gerald M Rubin, Teri-TB Ngo, David Shepherd, Christine Murphy, Heather Dionne, Barret D Pfeiffer, Amanda Cavallaro, Donald Hall, Jennifer Jeter, Nirmala Iyer, Dona Fetter, Joanna H Hausenfluck, Hanchuan Peng, Eric T Trautman, Robert R Svirskas, Eugene W Myers, Zbigniew R Iwinski, Yoshinori Aso, Gina M DePasquale, Adrianne Enos, Phuson Hulamm, Shing Chun Benny Lam, Hsing-Hsi Li, Todd R Laverty, Fuhui Long, Lei Qu, Sean D Murphy, Konrad Rokicki, Todd Safford, Kshiti Shaw, Julie H Simpson, Allison Sowell, Susana Tae, Yang Yu, Christopher T Zugates

2. publication:

   • Title: The neuronal architecture of the mushroom body provides a logic for associative learning
   • URL: https://doi.org/10.7554/eLife.04577
   • AuthorName: Yoshinori Aso, Daisuke Hattori, Yang Yu, Rebecca M Johnston, Nirmala A Iyer, Teri-TB Ngo, Heather Dionne, LF Abbott, Richard Axel, Hiromu Tanimoto, Gerald M Rubin

3. publication:

   • Title: Color depth MIP mask search: a new tool to expedite Split-GAL4 creation
   • URL: https://doi.org/10.1101/318006
   • AuthorName: Hideo Otsuna, Masayoshi Ito, Takashi Kawase

4. publication:

   • Title: An unbiased template of the Drosophila brain and ventral nerve cord
   • URL: https://doi.org/10.1101/376384
   • AuthorName: John A Bogovic, Hideo Otsuna, Larissa Heinrich, Masayoshi Ito, Jennifer Jeter, Geoffrey Meissner, Aljoscha Nern, Jennifer Colonell, Oz Malkesman, Kei Ito, Stephan Saalfeld

5. publication:

   • Title: An image resource of subdivided Drosophila GAL4-driver expression patterns for neuron-level searches
   • URL: https://doi.org/10.1101/2020.05.29.080473
   • AuthorName: Geoffrey W Meissner, Zachary Dorman, Aljoscha Nern, Kaitlyn Forster, Theresa Gibney, Jennifer Jeter, Lauren Johnson, Yisheng He, Kelley Lee, Brian Melton, Brianna Yarbrough, Jody Clements, Cristian Goina, Hideo Otsuna, Konrad Rokicki, Robert R Svirskas, Yoshinori Aso, Gwyneth M Card, Barry J Dickson, Erica Ehrhardt, Jens Goldammer, Masayoshi Ito, Wyatt Korff, Ryo Minegishi, Shigehiro Namiki, Gerald M Rubin, Gabriella Sterne, Tanya Wolff, Oz Malkesman, FlyLight Project Team