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      Neuron action potentials were visualized using the voltage-sensitive fluorescent
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      HEPES (pH 7.3, 290-300 mOsm/kg). The exposure system comprised a Siglent SDG1032X
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      precisely positioned 50 µm above a chosen neuron using a robotic micromanipulator
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      generated using Excel software, uploaded to the Siglent generator, and exposure
      parameters (frequency, amplitude, and duration) were set. Exposure was triggered
      50 ms after the start of fluorescence time-lapse recording. The applied electric
      field between electrodes was determined using a finite element method simulation
      (Sim4Life V5.2, Zurich Med Tech). Exposure duration was kept at 250 ms regardless
      of the sine wave carrier and modulation frequency. Waveform, amplitude, and synchronization
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      HEPES (pH 7.3, 290-300 mOsm/kg). The exposure system comprised a Siglent SDG1032X
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      precisely positioned 50 µm above a chosen neuron using a robotic micromanipulator
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      50 ms after the start of fluorescence time-lapse recording. The applied electric
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      of the sine wave carrier and modulation frequency. Waveform, amplitude, and synchronization
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      Neuron action potentials were visualized using the voltage-sensitive fluorescent
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      HEPES (pH 7.3, 290-300 mOsm/kg). The exposure system comprised a Siglent SDG1032X
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      precisely positioned 50 µm above a chosen neuron using a robotic micromanipulator
      MPC-200 (Sutter Instrument, Novato, CA). Sine waves and modulated sine waves were
      generated using Excel software, uploaded to the Siglent generator, and exposure
      parameters (frequency, amplitude, and duration) were set. Exposure was triggered
      50 ms after the start of fluorescence time-lapse recording. The applied electric
      field between electrodes was determined using a finite element method simulation
      (Sim4Life V5.2, Zurich Med Tech). Exposure duration was kept at 250 ms regardless
      of the sine wave carrier and modulation frequency. Waveform, amplitude, and synchronization
      were monitored with a TDS3052C oscilloscope (Tektronix, Beaverton, OR). Exposures
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      electrode configuration, 1V translates into 36 V/cm at the location of neurons.
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      system comprised a Siglent SDG1032X waveform generator (Siglent Technologies,
      Solon, OH), the ''EasywaveX'' software, and a pair of tungsten electrodes (100
      μm diameter, 140 μm inter-electrode gap) precisely positioned 50 µm above a chosen
      neuron using a robotic micromanipulator MPC-200 (Sutter Instrument, Novato, CA).
      Sine waves and modulated sine waves were generated using Excel software, uploaded
      to the Siglent generator, and exposure parameters (frequency, amplitude, and duration)
      were set. Exposure was triggered 50 ms after the start of fluorescence time-lapse
      recording. The applied electric field between electrodes was determined using
      a finite element method simulation (Sim4Life V5.2, Zurich Med Tech). Exposure
      duration was kept at 250 ms regardless of the sine wave carrier and modulation
      frequency. Waveform, amplitude, and synchronization were monitored with a TDS3052C
      oscilloscope (Tektronix, Beaverton, OR). Exposures were synchronized with image
      acquisitions via a TTL pulse protocol using a Digidata 1440A board and Clampex
      v. 10.2 software (Molecular Devices, Sunnyvale, CA).'
    identifier: 2kHz_spindle_stimulation_6V
    name: 2kHz_spindle_stimulation_6V
    schemaKey: Session
    startDate: '2024-05-09T22:32:58-04:00'
  - description: Metadata generated by DANDI cli
    endDate: '2024-05-25T20:52:07.187115-04:00'
    id: urn:uuid:5b90742e-7b6d-456f-b70f-47051028fb74
    name: Metadata generation
    schemaKey: Activity
    startDate: '2024-05-25T20:52:06.907762-04:00'
    wasAssociatedWith:
    - identifier: RRID:SCR_019009
      name: DANDI Command Line Interface
      schemaKey: Software
      url: https://github.com/dandi/dandi-cli
      version: 0.62.0