dandiarchive-dataset/s3://dandiarchive/dandisets/001032/draft/assets.yaml

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      HEPES (pH 7.3, 290-300 mOsm/kg). The exposure system comprised a Siglent SDG1032X
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      precisely positioned 50 µm above a chosen neuron using a robotic micromanipulator
      MPC-200 (Sutter Instrument, Novato, CA). Sine waves and modulated sine waves were
      generated using Excel software, uploaded to the Siglent generator, and exposure
      parameters (frequency, amplitude, and duration) were set. Exposure was triggered
      50 ms after the start of fluorescence time-lapse recording. The applied electric
      field between electrodes was determined using a finite element method simulation
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      of the sine wave carrier and modulation frequency. Waveform, amplitude, and synchronization
      were monitored with a TDS3052C oscilloscope (Tektronix, Beaverton, OR). Exposures
      were synchronized with image acquisitions via a TTL pulse protocol using a Digidata
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      Neuron action potentials were visualized using the voltage-sensitive fluorescent
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      HEPES (pH 7.3, 290-300 mOsm/kg). The exposure system comprised a Siglent SDG1032X
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      precisely positioned 50 µm above a chosen neuron using a robotic micromanipulator
      MPC-200 (Sutter Instrument, Novato, CA). Sine waves and modulated sine waves were
      generated using Excel software, uploaded to the Siglent generator, and exposure
      parameters (frequency, amplitude, and duration) were set. Exposure was triggered
      50 ms after the start of fluorescence time-lapse recording. The applied electric
      field between electrodes was determined using a finite element method simulation
      (Sim4Life V5.2, Zurich Med Tech). Exposure duration was kept at 250 ms regardless
      of the sine wave carrier and modulation frequency. Waveform, amplitude, and synchronization
      were monitored with a TDS3052C oscilloscope (Tektronix, Beaverton, OR). Exposures
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      electrode configuration, 1V translates into 36 V/cm at the location of neurons.
      Neuron action potentials were visualized using the voltage-sensitive fluorescent
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      containing (in mM): 140 NaCl, 5.4 KCl, 2 CaCl2, 1.5 MgCl2, 10 D-glucose, and 10
      HEPES (pH 7.3, 290-300 mOsm/kg). The exposure system comprised a Siglent SDG1032X
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      precisely positioned 50 µm above a chosen neuron using a robotic micromanipulator
      MPC-200 (Sutter Instrument, Novato, CA). Sine waves and modulated sine waves were
      generated using Excel software, uploaded to the Siglent generator, and exposure
      parameters (frequency, amplitude, and duration) were set. Exposure was triggered
      50 ms after the start of fluorescence time-lapse recording. The applied electric
      field between electrodes was determined using a finite element method simulation
      (Sim4Life V5.2, Zurich Med Tech). Exposure duration was kept at 250 ms regardless
      of the sine wave carrier and modulation frequency. Waveform, amplitude, and synchronization
      were monitored with a TDS3052C oscilloscope (Tektronix, Beaverton, OR). Exposures
      were synchronized with image acquisitions via a TTL pulse protocol using a Digidata
      1440A board and Clampex v. 10.2 software (Molecular Devices, Sunnyvale, CA).'
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      electrode configuration, 1V translates into 36 V/cm at the location of neurons.
      Neuron action potentials were visualized using the voltage-sensitive fluorescent
      dye, FluoVolt. All experiments were performed in a standard physiological solution
      containing (in mM): 140 NaCl, 5.4 KCl, 2 CaCl2, 1.5 MgCl2, 10 D-glucose, and 10
      HEPES (pH 7.3, 290-300 mOsm/kg). The exposure system comprised a Siglent SDG1032X
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      and a pair of tungsten electrodes (100 μm diameter, 140 μm inter-electrode gap)
      precisely positioned 50 µm above a chosen neuron using a robotic micromanipulator
      MPC-200 (Sutter Instrument, Novato, CA). Sine waves and modulated sine waves were
      generated using Excel software, uploaded to the Siglent generator, and exposure
      parameters (frequency, amplitude, and duration) were set. Exposure was triggered
      50 ms after the start of fluorescence time-lapse recording. The applied electric
      field between electrodes was determined using a finite element method simulation
      (Sim4Life V5.2, Zurich Med Tech). Exposure duration was kept at 250 ms regardless
      of the sine wave carrier and modulation frequency. Waveform, amplitude, and synchronization
      were monitored with a TDS3052C oscilloscope (Tektronix, Beaverton, OR). Exposures
      were synchronized with image acquisitions via a TTL pulse protocol using a Digidata
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      and will be reported separately after the calibration is completed). For the employed
      electrode configuration, 1V translates into 36 V/cm at the location of neurons.
      Neuron action potentials were visualized using the voltage-sensitive fluorescent
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      containing (in mM): 140 NaCl, 5.4 KCl, 2 CaCl2, 1.5 MgCl2, 10 D-glucose, and 10
      HEPES (pH 7.3, 290-300 mOsm/kg). The exposure system comprised a Siglent SDG1032X
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      and a pair of tungsten electrodes (100 μm diameter, 140 μm inter-electrode gap)
      precisely positioned 50 µm above a chosen neuron using a robotic micromanipulator
      MPC-200 (Sutter Instrument, Novato, CA). Sine waves and modulated sine waves were
      generated using Excel software, uploaded to the Siglent generator, and exposure
      parameters (frequency, amplitude, and duration) were set. Exposure was triggered
      50 ms after the start of fluorescence time-lapse recording. The applied electric
      field between electrodes was determined using a finite element method simulation
      (Sim4Life V5.2, Zurich Med Tech). Exposure duration was kept at 250 ms regardless
      of the sine wave carrier and modulation frequency. Waveform, amplitude, and synchronization
      were monitored with a TDS3052C oscilloscope (Tektronix, Beaverton, OR). Exposures
      were synchronized with image acquisitions via a TTL pulse protocol using a Digidata
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      the calibration is completed). For the employed electrode configuration, 1V translates
      into 36 V/cm at the location of neurons. Neuron action potentials were visualized
      using the voltage-sensitive fluorescent dye, FluoVolt. All experiments were performed
      in a standard physiological solution containing (in mM): 140 NaCl, 5.4 KCl, 2
      CaCl2, 1.5 MgCl2, 10 D-glucose, and 10 HEPES (pH 7.3, 290-300 mOsm/kg). The exposure
      system comprised a Siglent SDG1032X waveform generator (Siglent Technologies,
      Solon, OH), the ''EasywaveX'' software, and a pair of tungsten electrodes (100
      μm diameter, 140 μm inter-electrode gap) precisely positioned 50 µm above a chosen
      neuron using a robotic micromanipulator MPC-200 (Sutter Instrument, Novato, CA).
      Sine waves and modulated sine waves were generated using Excel software, uploaded
      to the Siglent generator, and exposure parameters (frequency, amplitude, and duration)
      were set. Exposure was triggered 50 ms after the start of fluorescence time-lapse
      recording. The applied electric field between electrodes was determined using
      a finite element method simulation (Sim4Life V5.2, Zurich Med Tech). Exposure
      duration was kept at 250 ms regardless of the sine wave carrier and modulation
      frequency. Waveform, amplitude, and synchronization were monitored with a TDS3052C
      oscilloscope (Tektronix, Beaverton, OR). Exposures were synchronized with image
      acquisitions via a TTL pulse protocol using a Digidata 1440A board and Clampex
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  dateModified: '2024-05-25T20:39:58.039182-04:00'
  digest:
    dandi:dandi-etag: 103e282ea23d137f7c8730edb0484fd7-1
    dandi:sha2-256: 13424e92a2ac1723ce1738d7bcc3bf9ac29c40965abd4f0fa59ec48d70cb9ba7
  encodingFormat: application/x-nwb
  id: dandiasset:72947b18-65e8-4798-9ce0-441741f941c1
  identifier: 72947b18-65e8-4798-9ce0-441741f941c1
  measurementTechnique: []
  path: sub-9-1/sub-9-1_ses-2kHz-spindle-stimulation-5V_image.nwb
  schemaKey: Asset
  schemaVersion: 0.6.7
  variableMeasured: []
  wasAttributedTo:
  - age:
      schemaKey: PropertyValue
      unitText: ISO-8601 duration
      value: P18D
      valueReference:
        schemaKey: PropertyValue
        value: dandi:BirthReference
    identifier: '9_1'
    schemaKey: Participant
    sex:
      name: Unknown
      schemaKey: SexType
    species:
      identifier: http://purl.obolibrary.org/obo/NCBITaxon_10116
      name: Rattus norvegicus - Norway rat
      schemaKey: SpeciesType
  wasGeneratedBy:
  - description: 'Subject ID: 9_1. A spindle (sine wave modulated at 20Hz frequency)
      stimulation applied at 2kHz using 5V (peak-to-peak) amplitude. Note: this is not
      the actual amplitude measured on the oscilloscope, which will be somewhat smaller
      (calibration factors are frequency-dependent and will be reported separately after
      the calibration is completed). For the employed electrode configuration, 1V translates
      into 36 V/cm at the location of neurons. Neuron action potentials were visualized
      using the voltage-sensitive fluorescent dye, FluoVolt. All experiments were performed
      in a standard physiological solution containing (in mM): 140 NaCl, 5.4 KCl, 2
      CaCl2, 1.5 MgCl2, 10 D-glucose, and 10 HEPES (pH 7.3, 290-300 mOsm/kg). The exposure
      system comprised a Siglent SDG1032X waveform generator (Siglent Technologies,
      Solon, OH), the ''EasywaveX'' software, and a pair of tungsten electrodes (100
      μm diameter, 140 μm inter-electrode gap) precisely positioned 50 µm above a chosen
      neuron using a robotic micromanipulator MPC-200 (Sutter Instrument, Novato, CA).
      Sine waves and modulated sine waves were generated using Excel software, uploaded
      to the Siglent generator, and exposure parameters (frequency, amplitude, and duration)
      were set. Exposure was triggered 50 ms after the start of fluorescence time-lapse
      recording. The applied electric field between electrodes was determined using
      a finite element method simulation (Sim4Life V5.2, Zurich Med Tech). Exposure
      duration was kept at 250 ms regardless of the sine wave carrier and modulation
      frequency. Waveform, amplitude, and synchronization were monitored with a TDS3052C
      oscilloscope (Tektronix, Beaverton, OR). Exposures were synchronized with image
      acquisitions via a TTL pulse protocol using a Digidata 1440A board and Clampex
      v. 10.2 software (Molecular Devices, Sunnyvale, CA).'
    identifier: 2kHz_spindle_stimulation_5V
    name: 2kHz_spindle_stimulation_5V
    schemaKey: Session
    startDate: '2024-05-09T22:32:28-04:00'
  - description: Metadata generated by DANDI cli
    endDate: '2024-05-25T20:39:58.039182-04:00'
    id: urn:uuid:34c7591b-03c6-48d7-98e1-b7600d8f702f
    name: Metadata generation
    schemaKey: Activity
    startDate: '2024-05-25T20:39:57.541261-04:00'
    wasAssociatedWith:
    - identifier: RRID:SCR_019009
      name: DANDI Command Line Interface
      schemaKey: Software
      url: https://github.com/dandi/dandi-cli
      version: 0.62.0