dandiarchive-dataset/s3://dandiarchive/dandisets/000448/draft/dandiset.yaml

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citation: Silkuniene, Giedre; Kim, Vitalii; Semenov, Iurii; Pakhomov, Andrei (2023)
  Time kinetics of the membrane potential at the cathode- and anode-facing poles of
  a cell induced by a train of 5 pulses at 833 kHz (Version draft) [Data set]. DANDI
  archive. https://dandiarchive.org/dandiset/000448/draft
contributor:
- affiliation: []
  email: gsilkuni@odu.edu
  identifier: 0000-0001-8436-9718
  includeInCitation: true
  name: Silkuniene, Giedre
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- affiliation: []
  identifier: 0000-0003-0699-5582
  includeInCitation: true
  name: Kim, Vitalii
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- affiliation: []
  identifier: 0000-0002-0302-1355
  includeInCitation: true
  name: Semenov, Iurii
  roleName: []
  schemaKey: Person
- affiliation: []
  identifier: 0000-0003-3816-3860
  includeInCitation: true
  name: Pakhomov, Andrei
  roleName: []
  schemaKey: Person
- awardNumber: 1R21EY034258
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  includeInCitation: false
  name: National Institutes of Health
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dateCreated: '2023-02-17T18:25:05.767450+00:00'
description: Time-lapse recordings for the investigation of the relationship between
  the electroporation efficiency of nsEP and changes in the transmembrane potential
  (TMP). A strobe imaging synchronized with nsEP exposure was used.  This allowed
  studying the nanosecond kinetics of TMP charging and relaxation, which is the primary
  effect of nsEP that rapidly charges the plasma membrane. The methodology involved
  pulsed laser fluorescence microscopy with voltage-sensitive FluoVolt dye-loaded
  cells that respond to TMP changes within nanoseconds. FluoVolt has a high sensitivity
  of about 10 % deltaF/F per 100 mV. During the imaging, a single short pulse laser
  flash is delivered at a precise time interval (addition of 25ns in each frame) before,
  during, or after nsEP exposure. The camera shutter opens in advance of and closes
  after the laser flash, capturing one TMP image per nsEP exposure. Supported by NIH
  1R21EY034258
ethicsApproval: []
id: DANDI:000448/draft
identifier: DANDI:000448
keywords: []
license:
- spdx:CC-BY-4.0
manifestLocation:
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name: Time kinetics of the membrane potential at the cathode- and anode-facing poles
  of a cell induced by a train of 5 pulses at 833 kHz
protocol: []
relatedResource:
- name: Action spectra and mechanisms of (in) efficiency of bipolar electric pulses
    at electroporation
  relation: dcite:IsPublishedIn
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  url: https://doi.org/10.1016/j.bioelechem.2022.108319
repository: https://dandiarchive.org
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url: https://dandiarchive.org/dandiset/000448/draft
version: draft
wasGeneratedBy:
- identifier: R21EY034258
  name: Targeted Neuromodulation by Nanosecond Pulsed Electric Fields
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